ASP Page not applying css and images

If web.config file entry below does not work:

<location path="images">
    <system.web>
      <authorization>
        <allow users="*" />
      </authorization>
    </system.web>
  </location>
  <location path="css">
    <system.web>
      <authorization>
        <allow users="*" />
      </authorization>
    </system.web>
  </location>

     
Open up IIS manager and click authentication and set anonymous to application pool if your using Integrated Authentication.